Journal: Journal of insect physiology

Article Title: Neurochemical regulation of Aedes aegypti salivary gland function.

doi: 10.1016/j.jinsphys.2021.104193

Figure Lengend Snippet: Fig. 5. Immunolocalization of DA receptor in the isolated A. aegypti salivary gland with rat D1- receptor polyclonal antibody. (A) overview of sali vary glands at low magnification with the labels for salivary duct (SD), lateral lobes (LL), proximal lateral lobes (PLL), distal lateral lobes (DLL), median lobe (ML). Square insert in panel A is a representative image (n = 5) of gland treated with secondary anti body only (negative control). Hashtags correspond to the location of the magnified image for the median lobe (B), dorsal lateral lobe (C), proximal lateral lobe (D). Positive staining (green) of the salivary gland with the human D1 antibody was observed in different patterns for all lobes. DAPI counterstaining (blue) was used to localize nuclei. Scale bars are specific for each panel and images are representative of 5 individual replicates. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: After washing, the glands were incubated with rat anti-D1 or anti-D2 dopamine receptor polyclonal antibody at a dilution factor of 1:50 (Alamone Labs, Jerusalem, Israel) for 24 h at 4◦.

Techniques: Isolation, Negative Control, Staining

Journal: Journal of insect physiology

Article Title: Neurochemical regulation of Aedes aegypti salivary gland function.

doi: 10.1016/j.jinsphys.2021.104193

Figure Lengend Snippet: Fig. 6. Immunolocalization of DA receptor in the isolated A. aegypti salivary gland with rat D2- receptor polyclonal antibody. (A) overview of sali vary glands at low magnification with the labels for salivary duct (SD), lateral lobes (LL), proximal lateral lobes (PLL), distal lateral lobes (DLL), median lobe (ML). Hashtags correspond to the location of the magnified image for the median lobe (B), dorsal lateral lobe (C), proximal lateral lobe (D). Positive staining (green) of the salivary gland with the human D2 antibody was observed in different patterns for all lobes. DAPI counterstaining (blue) was used to localize nuclei. Scale bars are specific for each panel and images are representative of 5 individual repli cates. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: After washing, the glands were incubated with rat anti-D1 or anti-D2 dopamine receptor polyclonal antibody at a dilution factor of 1:50 (Alamone Labs, Jerusalem, Israel) for 24 h at 4◦.

Techniques: Isolation, Staining

Journal: Reproductive biomedicine online

Article Title: Social psychogenic stress promotes the development of endometriosis in mouse.

doi: 10.1016/j.rbmo.2016.11.012

Figure Lengend Snippet: Figure 4 – Immmunoreactivity staining of different markers in ectopic lesions in different groups. (A) Representative immunostaining of ADRB2 in endometrium in CONTROL and SHAM mice and in ectopic endometrium in STRESSED and UNSTRESSED mice. ADRB2 and DRD2 immunoreactivity was both seen primarily in glandular epithelial cells and was localized in the cytoplasm. Scale bar = 125 μm. (B) Representative immunostaining of DRD2, VEGF, CD31, CD41, F4/80, PCNA and α-SMA in the ectopic lesions in UNSTRESSED and STRESSED groups. VEGF immunoreactivity was seen primarily in glandular epithelial cells and was localized in the cytoplasm. CD31 immunostainings were seen mostly in vascular endothelial cells. CD41 shows the extent of platelet aggregation and F4/80 immunoreactivity represents the extent of macrophage infiltration. PCNA immunoreactivity was seen both in glandular epithelial cells and stromal cells were localized in the cell nucleus, but the change of immunoreactivity in glandular epithelial cells was more obvious. α-SMA staining were seen mostly in the stromal component of the ectopic lesions. Scale bar = 125 μm. Mice in stressed (STRS) and unstressed (UNSTRS) groups had undergone endometriosis-inducing surgery, while mice the SHAM group underwent non-endometriosis-inducing surgery. Mice in unstressed (UNSTRS), SHAM and control (CTL) groups were not exposed to stress.

Article Snippet: For negative controls, the immunoglobulin G (IgG) from the rabbit serum (Sigma, Darmstadt, Germany) was used instead of primary antibodies against ADRB2, DRD2, VEGF, CD31, CD41, α-SMA and PCNA except for F4/80, for which the IgG was from the rat serum (Boster, Wuhan, China) was used, all used at the same concentration with its corresponding primary antibody.

Techniques: Staining, Immunostaining, Control